An ACQ file is a data graph file created by AcqKnowledge, a program used to simulate physiological data recorded from different systems and transducers. It contains physiological data recorded from an organism that is displayed in waveforms in AcqKnowledge. ACQ files also store information about how the data was collected, including the rate and duration of the collection.

ACQ files, along with GTL graph template file are the main file types associated with the AcqKnowledge program. You can created an ACQ file by selecting File → Save as and choosing "ACQ". Once the file is created you can modify the file, save the file, or export the file to another format. NOTE: AcqKnowledge is commonly used with BIOPAC hardware, which specializes in collecting data such as EMG, pulse, respiration, EEG, electrogastrogram, temperature, and eye movement of a person.